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Cats Engineered for Disease Resistance

Cats Engineered for Disease Resistance

by: Ashley Beyer

As reported in the online Science News article called “Cats Engineered for Disease Resistance” by Tina Hesman Saey on September 12, 2011 (found at:
http://www.sciencenews.org/view/generic/id/334271/title/Cats_engineered_for_disease_resistance) cats, which have been genetically modified, may be immune to the HIV-like
virus.

Worldwide, HIV has infected and killed many for over three decades. Still today, HIV can be slowed down, but there is no cure. In the feline species, the feline immunodeficiency virus
(FIV) is similar to HIV. Scientists have engineered cats with a gene, which makes an antiviral protein that will hopefully fight FIV. The scientists also add another gene that makes the cat glow green under fluorescent lights so they can see their results. Cats acquire this FIV virus because they lack TRIM5alpha, a key immune protein, which fights viruses before the rest of the immune system senses an infection. During the procedure, the researchers collected eggs of a cat left after spaying and injected the antiviral protein from the genes of a rhesus monkey and green fluorescent protein into the eggs. The viruses then inserted the genes into the DNA of the eggs. After fertilization, the researchers put the embryos into the wombs of the cat surrogate mothers. Three transgenic kittens, which are kittens with the foreign genes, were born. These cats were able to pass on the genes to their kittens, also. It is still questionable if these cats will be able to pass along any of the genes. The research team is continuing breeding the cats to test their immunity against the virus.

The leader of this study was Eric Poeschla, a molecular virologist at the Mayo Clinic in Rochester, Minnesota. Poeschla is driven to find a cure to FIV, which may lead to a cure
of HIV in people. He also thinks broadly; he studied a similar disease in cats and experimented with this to help find clues to cure HIV in humans. He is eager to volunteer and wants to help both the human and cat species. Poeschla and his team are both curious and obsessed in that they are continuing their study with continuing breeding the cats and doing more research. The researchers are visionaries in the hope of one day finding the cure to HIV.

This study is important since this may be a possible clue to help fight HIV infections in people. Also, at the very least, the experiment found a way to help fight against FIV in cats. HIV has been around since the 1980s and has killed more than 25 million people. Society would highly benefit if AIDS could be cured. The death rate from this
virus would be significantly lower and it would be a step forward in the scientific community. In Africa, for example, the population would greatly increase if HIV was cured. In addition, this research may lead to similar experiments in different species of animals with FIV-like viruses. It should be reassuring to society that scientists are still diligently working to find cures to different diseases, even though they haven’t always been successful.

438 Responses to “Cats Engineered for Disease Resistance”

  1. Ade Ilesanmi says:

    Researchers filter through AIDS litter–still clawing for more answers

    Based on information as reported by Ashley Beyer from “Cats Engineered for Disease Resistance” by Tina Hesman Saey on September 12, 2011 (found at http://www.sciencenews.org/view/generic/id/334271/title/Cats_engineered_for_disease_resistance) and from “Transgenic Animals: An Interactive Qualifying Project” by M. Bradley (found at http://www.wpi.edu/Pubs/E-project/Available/E-project-082306…/IQP.pdf)
    The recent attempts by Eric Poeschla and his team to unlock the mysteries of the AIDS virus through use of transgenic cats certainly are not the first of their kinds. Chimpanzees were initially the go-to animals for AIDS models, which is logical considering that SIV (simian immunodeficieny virus, a.k.a. African Green Monkey Virus, a.k.a. Monkey AIDS) was first transmitted from our hunted primate friends to their African human hunters, most likely through skinning and cutting processes. AIDS researches soon realized that using chimp models is inefficient, as chimps are rare and, therefore, expensive. Also, because chimps do not progress from the HIV stage to the AIDS stage, the research that can be conducted on them is relatively limited. Mouse models soon became a better replacement; though, ironically, before the transgenic AIDS mouse, mice were not suitable models for AIDS vaccines and treatments because they cannot be infected with HIV. Their cells lack one or more of the factors necessary for in-host HIV replication. Mice cells have, therefore, been modified to express the receptor, co-receptor, and three human proteins needed for the vaccine to attack and replicate successfully in the cell. Regardless, the cells were unsuitable habitats for HIV.
    Which is why this new research revealing the cat as a potential model animal for AIDS researchers has rightfully excited AIDS researchers like Poeschla. Obviously, FIV and HIV are not mutually interchangeable viruses, but if cats can be genetically altered to express the protein necessary to fight off their version of HIV, then perhaps the homologous gene in humans can be found and made to express the human version of the “key immune protein.” Granted, I’m sure there is more than one protein necessary for the attack of the FIV virus in cats, as many metabolic reactions occur in cascades. Perhaps Poeschela and his team would have more success in getting the FIV immunity to carry through subsequent feline generations if they focused on the gene family involved in the specific HIV attack mechanism they are studying. If all the factors of that mechanism were emphasized, not just the TRIM5alpha protein of current focus, but all the proteins expressed by genes similar to that of TRIM5alpha, then it’s possible that the immune response may be more potent and, thus, more viable in subsequent generations. This would be a big deal for humans, because if the homologous gene family could be identified and isolated in humans (it may be in a non-coding part of the human genome, for all we know) gene therapy could be used to create vaccines that could, at least, establish some degree of immunity to HIV.
    While I agree with my colleague in her assessment that Poeschla and his team are driven, I don’t think their behavior necessarily qualifies as obsessed just because they want to continue their research by breeding more cats. It’s simply part of the scientific method–you see what isn’t working, and you keep trying to find the solution. While this study opens up a new door for AIDS research, I think it is far from any type of cure; though, findings from Poeschla’s team’s study will probably be useful to other researchers in other fields of AIDS research, and vice versa, and their findings, together, will probably be closer to identifying the cure (or cures) than one set of findings, alone.

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